Alpha-Klotho (Soluble) ELISA 27998 from IBL- America

Product Specs
ItemAlpha-Klotho (Soluble) ELISA
CompanyIBL- America
Price Pricing Info Supplier Page View Company Product Page
Catalog Number27998
Detection Range0 / 93.75 - 6000 pg/mL
Sensitivity6.15 pg/mL
Volume100 µL of properly diluted unknown / determination
Sample TypeSerum, plasma (EDTA, Heparin, Citrate), urine and cell culture supernatant
Regulatory StatusFor research use only, not for use in diagnostic procedures
ApplicationsELISA Kit
Quantity96 Determinations
ReactivityHuman
Assay Time1 hour incubation (RT) + 30 min. (RT) + 30 min. (RT) = 2 hours total incubation time
Storage2 - 8 °C

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IBL- America

8201 Central Ave NE Suite P

Minneapolis, Minnesota 55432

United States

Phone: 888-523-1246
763-780-2988

Company Profile

Website: http://www.ibl-america.com/

(1) Relationship between klotho and physical function in healthy agingScientific ReportsNovember 30, 2023Eliott Arroyo, Cecilia A. Leber, Heather N. Burney, Gayatri Narayanan, Ranjani Moorthi, Keith G. Avin, Stuart J. Warden, Sharon M. Moe, Kenneth Limserum using a well-validated solid-phase sandwich enzyme-linked immunosorbent assay (ELISA) (Cat. No. 27998, Immuno-Biological Laboratories, Inc., Minneapolis, MN, USA)27. To eliminate inter-assay variance, all

(2) A Combined Biomarker That Includes Plasma Fibroblast Growth Factor 23, Erythropoietin, and Klotho Predicts Short- and Long-Term Morbimortality and Development of Chronic Kidney Disease in Critical Care Patients with Sepsis: A Prospective CohortBiomoleculesOctober 3, 2023Luis Toro, Verónica Rojas, Carolina Conejeros, Patricia Ayala, Alfredo Parra-Lucares, Francisca Ahumada, Paula Almeida, María Fernanda Silva, Karin Bravo, Catalina Pumarino, et al.FGF23: 60-6600, Quidel Immutopics, San Diego, CA; EPO: DEP00, R&D Systems, Minneapolis, MN. Klotho: 27998, IBL-America, Minneapolis, MN, USA) according to manufacturer’s specifications. The inter-assay coefficient

(3) Birth size and the serum level of biological age markers in menScientific ReportsAugust 30, 2023Agnieszka Żelaźniewicz, Judyta Nowak-Kornicka, Bogusław Pawłowskilevel was measured with enzyme-linked immunosorbent assay (ELISA) using commercial kits (IBL® Code no 27998; with inter-assay and intra-assay coefficient of variation less than 11.4% and less than 3.5% respectively

(4) Serum α-Klotho associated with oral health among a nationally representative sample of US adultsFront Endocrinol (Lausanne)September 20, 2022Guo-Qiang Chen, Yao Duan, Jin-Feng Wang, Ying Lian, Xiu-Li Yinprotocol, serum α-klotho was assayed using a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA 27998, IBL International, Japan). This assay is reported to have a sensitivity of 6 pg/mL. All sample analyses

(5) Serum α-KL, a potential early marker of diabetes complications in youth with T1D, is regulated by miRNA 192Front Endocrinol (Lausanne)August 5, 2022Zhenwei Gong, Pedro A. Pagán Banchs, Ye Liu, Haoyi Fu, Vincent C. Arena, Erick Forno, Ingrid Libman, Jacqueline Ho, Radhika MuzumdarNumber: PRO15100286). Serum KL level Serum α-KL level was determined using ELISA kit from IBL (Cat# 27998, Immuno-Biological Labs, Japan) according to the manufacturer’s instructions. The coefficient of variability

(6) Gender-specific soluble α-klotho levels as marker of GH deficiency in children: a case–control studyJournal of Endocrinological InvestigationJune 1, 2022V. Guarnotta, G. Pizzolanti, R. Petrancosta, S. Radellini, C. Baiamonte, C. Giordanoanalyzed using a commercial solid phase sandwich ELISA (Enzyme-linked Immunosorbent Assay) assay kit (cat.27998, Immuno-Biological Laboratoires Co., Fujioka-Shi, Japan). The normal measurement range was 93.75–6000

(7) Orally-active, clinically-translatable senolytics restore α-Klotho in mice and humansEBioMedicineMarch 1, 2022Zhu Y, Prata LGPL, Gerdes EOW, Netto JME, Pirtskhalava T, Giorgadze N, Tripathi U, Inman CL, Johnson KO, Xue A, et al.diluted mouse urine (IBL America, #27601). Human urine α-Klotho was assayed by ELISA (IBL America, #27998, RRID:AB_2750859). Urine α-Klotho measurements are expressed as a function of urine volume, creatinine

(8) S-Klotho level and physiological markers of cardiometabolic risk in healthy adult menAging (Albany NY)January 31, 2022Agnieszka Żelaźniewicz, Judyta Nowak-Kornicka, Bogusław Pawłowskilevel was measured with enzyme-linked immunosorbent assay (ELISA) using commercial kits (IBL® Code no 27998; with inter-assay and intra-assay coefficient of variation less than 11.4% and less than 3,5% respectively

(9) Epigenetic and senescence markers indicate an accelerated ageing-like state in women with preeclamptic pregnanciesEBioMedicineAugust 11, 2021Sonja Suvakov et al.concentrations were measured using the Alpha Klotho Human Soluble ELISA Assay kit (IBL-America. Inc, Cat#27998). α-Klotho was normalized to urinary creatinine concentration measured using a creatinine assay kit

(10) Assessment of Clinical Utility of Assaying FGF-23, Klotho Protein, Osteocalcin, NTX, and Sclerostin in Patients with Primary HyperparathyroidismJournal of Clinical MedicineJuly 13, 2021Monika Sykała et al.soluble α-Klotho assay kit—IBL (Immuno-Biological Laboratories Co. (Minneapolis, MN, USA); code no. 27998); minimum detectable concentration: 6.15 pg/mL; CV = 2.7%.Osteocalcin: enzyme-linked immunosorbert assay

(11) A novel antibody for the detection of alternatively spliced secreted KLOTHO isoform in human plasmaPloS oneJanuary 1, 2021Jadhav S, Tripathi S, Chandrekar A, Waikar SS, Hsiao LL.failed to detect rsKL. We next tested the most commonly used commercially available ELISA kit from IBL (#27998, Immuno-biological PLOS ONE | https://doi.org/10.1371/journal.pone.0245614 January 22, 2021 3/9 PLOS

(12) Fibroblast growth factor 23 decreases PDE4 expression in heart increasing the risk of cardiac arrhythmia; Klotho opposes these effectsBasic Research in CardiologySeptember 1, 2020Marta Lindner, Hind Mehel, Amandine David, Christine Leroy, Martine Burtin, Gérard Friedlander, Fabiola Terzi, Delphine Mika, Rodolphe Fischmeister, Dominique Priéconcentration of sKlotho was determined with the IBL Elisa kit (Human soluble α-Klotho Assay Kit code No. 27998 – IBL, Japan). 6 cAMP measurements by FRET. ARVMs were infected with an adenovirus encoding the

(13) Effect of kidney donation on bone mineral metabolismPLOS ONEJuly 7, 2020Thomas F. Hiemstra et al.of human FGF-23. Serum α-Klotho levels were analyzed (Immuno-Biological Laboratories Co., Ltd., Cat #27998). 25-Hydroxyvitamin D and 1,25-Dihydroxyvitamin D were analysed by automated chemiluminescent immunoassay

(14) Urinary Extracellular Vesicles Carrying Klotho Improve the Recovery of Renal Function in an Acute Tubular Injury ModelMolecular therapy : the journal of the American Society of Gene TherapyFebruary 5, 2020Grange C, Papadimitriou E, Dimuccio V, Pastorino C, Molina J, O'Kelly R, Niedernhofer LJ, Robbins PD, Camussi G, Bussolati B.quantification of Klotho levels within EVs was performed using an anti-human ELISA (Human soluble a-Klotho code 27998; Immuno-Biological Laboratories)73 according to the manufacturer’s protocol. In detail, three pools

(15) FGF23-klotho axis as predictive factors of fractures in type 2 diabetics with early chronic kidney diseaseJournal of Diabetes and its ComplicationsJanuary 1, 2020Ana Luísa Ribeiro, Filipa Mendes, Eduarda Carias, Fátima Rato, Nélio Santos, Pedro Leão Neves, Ana Paula Silva(Cat.#60-6100 Immutopics Inc., San Clemente, CA, USA) and Human soluble αKlotho ELISA kit (Code No. 27998, IBL – Immuno-Biological Laboratories Co., Ltd., Gunma, Japan), according to the manufacturer's instructions. The

(16) Plasmatic Klotho and FGF23 Levels as Biomarkers of CKD-Associated Cardiac Disease in Type 2 Diabetic PatientsInternational Journal of Molecular SciencesMarch 27, 2019Ana Paula Silva et al.Soluble α-Klotho serum levels were determined by ELISA using the Human soluble α-Klotho kit (Code no- 27998, IBL – Immuno-Biologial Laboratories Co., Ltd., Gunma, Japan), by adapting the manufacturer’s instructions

(17) Aldosterone induces renal fibrosis by promoting HDAC1 expression, deacetylating H3K9 and inhibiting klotho transcriptionMolecular Medicine ReportsDecember 20, 2018Lingyun Lai, Ping Cheng, Minhua Yan, Yong Gu, Jun Xuewas determined using an ELISA kits obtained from Immuno‑Biological Laboratories Co., Ltd. (cat. no. 27998; Fujioka, Japan), according to the manufacturer's instructions. ∆ aldosterone was calculated using the

(18) The Value of Older Donors' Klotho Level in Predicting Recipients' Short-Term Renal FunctionMedical science monitor : international medical journal of experimental and clinical researchNovember 5, 2018Deng G, Yang A, Wu J, Zhou J, Meng S, Zhu C, Wang J, Shen S, Ma J, Liu D.of recipients were also calculated. Serum Klotho (Immuno Biological Laboratories Co., Tokyo, Japan, 27998), intact FGF-23 (R&D Systems, Minneapolis, MN, USA, DY2604-05), and urinary NGAL (R&D Systems, Minneapolis,

(19) Multiple sclerosis influences on the augmentation of serum Klotho concentrationJournal of the Neurological SciencesMarch 15, 2016Mona Ahmadi, Mohammad Sajad Emami Aleagha, Mohammad Hossein Harirchian, Reza Yarani, Farhad Tavakoli, Bahaadin Siroosmeasured using a sandwich ELISA assay kit (human soluble αklotho assay kit, IBL Co., Japan, Code No. 27998 and Lot No. 1L-303). The sensitivity of kit was 6.15 pg/ml. The assay was performed according to the

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Alpha-Klotho (Soluble) ELISA from IBL- America

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IBL- America

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Alpha-Klotho (Soluble) ELISA from IBL- America

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